About Proteomics Core Facility

About Proteomics

The core aims to accommodate the research needs of the academic community and provides services, facilities, and expertise to any group seeking to explore proteomics.

We provide a wide array of services for the characterization of protein samples, ranging from discovery proteomics, quantitative Tandem Mass Tag analyses, gel band identification, post-translational modification analyses, co-immunoprecipitation analyses, and more. We also provide sample preparation and data analysis as part of our service.

Samples are processed and analyzed on a sensitive nanoflow liquid chromatography system coupled to a high-resolution Orbitrap Fusion mass spectrometer.

 

Equipment

The proteomics core is equipped with a Thermo Orbitrap Fusion Tribrid ETD mass spectrometer and Thermo Easy nanoLC 1200 liquid chromatography system. This setup allows sensitive and versatile high-resolution data acquisition.

The core also has a variety of equipment specifically for sample preparation and processing, including speed vacuum concentrators, a Precellys tissue/cell homogenizer, and centrifuges.

Data processing is performed on workstations using Proteome Discoverer.

Some peripheral equipment is available for use at an hourly rate by users, see the pricing page.

Instrumentation funding has been supported by NIH grant S10 OD010669

https://creativecommons.org/licenses/by-sa/3.0/

Orbitrap Fusion Lumos
Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer 
https://www.thermofisher.com/order/catalog/product/IQLAAEGAAPFADBMBHQ
EASY-nLC™ 1200 System
EASY-nLC™ 1200 System
https://assets.thermofisher.com/TFS-Assets/CMD/Specification-Sheets/PS-64582-LC-EASY-nLC-1200-PS64582-EN.pdf
ÄKTA™ go
ÄKTA™ go
https://www.cytivalifesciences.com/en/us/shop/chromatography/chromatography-systems/akta-go-protein-purification-system-p-11219
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